Research: Confocal Microscopy for Corneal Microscopic Foreign Bodies in Horses

Author:
Publish date:

The next issue of Veterinary Ophthalmology from the American College of Veterinary Ophthalmologists is now online for early view from Wiley. Included in this magazine is “In vivo confocal microscopy of corneal microscopic foreign bodies in horses.”

Abstract

Objective

To describe in vivo corneal confocal microscopy of horses with microscopic corneal foreign bodies and to correlate findings with clinical, cytological, and histopathologic evaluations of clinical cases and foreign body morphologies observed in vitro with the confocal microscope.

Animals studied

Five horses with microscopic corneal foreign bodies.

Procedures

Sedated and anesthetized horses were examined with a modified Heidelberg Retina Tomograph II and Rostock Cornea Module. Confocal microscopy images were compared with images from cytologic and histopathologic corneal samples. To establish microscopic morphologic features, confocal microscopy images of burdock pappus bristles and surgical glove powder were obtained by in vitro examination.

Results

Horses were examined by in vivo confocal microscopy to assist in identifying corneal opacities detected by slit-lamp biomicroscopy, to determine the etiology of clinically idiopathic keratitis, or to localize corneal opacities presumed to be foreign bodies for surgical planning. Corneal foreign bodies presumptively identified by confocal microscopy included burdock pappus bristles, other plant foreign materials, and surgical glove powder. The corneal foreign bodies appeared as moderately or hyper-reflective linear, circular, or oval structures by confocal microscopy and did not resemble any normal anatomic structures. The confocal microscopic identification of the foreign bodies was corroborated by cytologic and histopathologic findings in some horses. The in vivo confocal microscopic appearance of the foreign bodies was consistent with morphologies observed during examination of foreign bodies in vitro.

Conclusions

In vivo corneal confocal microscopy provides a noninvasive method for the detection, characterization, and localization of microscopic foreign bodies in the equine cornea.

Authors

Eric C. Ledbetter, Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA; Hospital for Animals, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA

Nita L. Irby, Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA

Deanna M. W. Schaefer, Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA